activity of catalase.

Aim: To investigate on the activity of catalase. Introduction Catalase, an enzyme institute in many different tissues, catalyses the breakdown of atomic number 1 peroxide into piss and atomic number 8. 2H₂O₂ → 2H₂O + O₂ atomic add up 1 peroxide is a toxic substance that sens be formed during aerobic respiration and catalase removes this product. The activity of catalase faeces be measured by finding the rate of oxygen release from henry peroxide. Minced murphy provides a suitable witnesser of catalase and the pH is varied in this experiment using citric acid-sodium inorganic phosphate buffer settlements at pH values of 4.4, 5.2, 6.5, 7.5 and 9. meditation: As the value of pH increases, the numerate of oxygen increases up to a point where the tot decreases. This is because the active site is denaturised and the 3 dimensional anatomy is altered. Apparatus: Citric acid-sodium phosphate buffer solutions of pH 4.4, 5.2, 6.5, 7 .5 and 9 Hydrogen peroxide solution Potato 10ml and 100ml measuring piston chamber Stopwatch base of operations and clamp Water bucket pH paper, pH time and pH probe connected to info logger.

Cork stone drill Large test tube Rubber stopper and preservation tube Risk judging/safety precautions Risk assessment sentry go precautions Citric acid may be irritant simulate gloves, lab coat, and safety gawp Oxidising hydrogen peroxide is corrosive go to pieces gloves, lab coat, and safety goggles Fragile glassware eg measuring piston chamberPlace glassware in the substance of the table Variables: Independent variant: pH level pendant multivariate: average v olume of oxygen produced Control variable: ! Same size of potato, same amount of hydrogen peroxide. modify and non destructive testing of material: Destructive testing: hydrogen peroxide, pH, potato Non destructive testing: pH meter does not alter the solution, test tube, measuring cylinder Method: 1.Obtained dick as shown below 2.Used a cork borer, cut cylinder of...If you want to get a full essay, order it on our website: OrderEssay.net

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